Acrolein (ACR) antibody

Antigen: Acrolein (ACR)

Clonality: Monoclonal (F56)

Host: Mouse

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN335409

Additional Information

Immunogen: ACR-modified keyhole-lympet hemocyanine

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: F56

Description: Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative)

Application Details

Application Notes: IHC: Recommended antibody concentration is 0.5-1.0 micro gram/mL on paraformaldehyde fixed tissue.

Buffer: Frozen (100 micro g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.

Storage: Less than 20°C.

Research Area: Drugs, Alzheimer's Disease, Neurology

Restrictions: For Research Use only

Images

Cross-reactivity of anti-ACR antibody

Publications

Publications: Uchida, Kanematsu, Sakai et al.: "Protein-bound acrolein: potential markers for oxidative stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 4882-7, 1998 (PubMed).

Calingasan, Uchida, Gibson: "Protein-bound acrolein: a novel marker of oxidative stress in Alzheimer's disease." in: Journal of neurochemistry, Vol. 72, Issue 2, pp. 751-6, 1999 (PubMed).

Shibata, Nagai, Miyata et al.: "Nonoxidative protein glycation is implicated in familial amyotrophic lateral sclerosis with superoxide dismutase-1 mutation." in: Acta neuropathologica, Vol. 100, Issue 3, pp. 275-84, 2000 (PubMed).

Shibata, Hirano, Hedley-Whyte et al.: "Selective formation of certain advanced glycation end products in spinal cord astrocytes of humans and mice with superoxide dismutase-1 mutation." in: Acta neuropathologica, Vol. 104, Issue 2, pp. 171-8, 2002 (PubMed).

Iuchi, Kaneko, Matsuki et al.: "Carbonyl stress and detoxification ability in the male genital tract and testis of rats." in: Histochemistry and cell biology, Vol. 121, Issue 2, pp. 123-30, 2004 (PubMed).

Zarkovic, Uchida, Kolenc et al.: "Tissue distribution of lipid peroxidation product acrolein in human colon carcinogenesis." in: Free radical research, Vol. 40, Issue 6, pp. 543-52, 2006 (PubMed).

Tamamizu-Kato, Wong, Jairam et al.: "Modification by acrolein, a component of tobacco smoke and age-related oxidative stress, mediates functional impairment of human apolipoprotein E." in: Biochemistry, Vol. 46, Issue 28, pp. 8392-400, 2007 (PubMed).