HNE antibody

Catalog No. ABIN335415

Product Details anti-HNE Antibody

Target: HNE (4-Hydroxynonenal (HNE))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Specific for 4HNE-Lysine adduct in all species. This antibody has almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.

Purification: Ammonium sulphate purified IgG

Immunogen: 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.

Clone: HNEJ-2

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, Western and/or ELISA. Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 200μL of distilled water.

Buffer: Lyophilized as 100 ug/mL IgG in 50 mM Tris buffered saline (TBS)

Storage: -20 °C

References for anti-HNE antibody (ABIN335415)

Tskitishvili, Sharentuya, Temma-Asano, Mimura, Kinugasa-Taniguchi, Kanagawa, Fukuda, Kimura, Tomimatsu, Shimoya: "Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells." in: Molecular human reproduction, Vol. 16, Issue 3, pp. 188-99, (2010) (PubMed).

Ogawa, Fujii, Yoshizato, Kawada: "A human-type nonalcoholic steatohepatitis model with advanced fibrosis in rabbits." in: The American journal of pathology, Vol. 177, Issue 1, pp. 153-65, (2010) (PubMed).

Wakamatsu, Dogru, Ayako, Takano, Matsumoto, Ibrahim, Okada, Satake, Fukagawa, Shimazaki, Tsubota, Fujishima: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, (2010) (PubMed).

Raz, Zhang, Zhou, Han, Gulati, Yang, Yang, Wang, Brann: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, (2010) (PubMed).

Kurashige, Abe, Furusu, Miyazaki, Obata, Xia, Nakazawa, Nakazawa, Funakoshi, Harada, Koji, Kohno: "Renoprotective effect of azelnidipine in rats." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 12, pp. 2237-44, (2008) (PubMed).

Amidi, French, Chung, Halsted, Medici, French: "M-30 and 4HNE are sequestered in different aggresomes in the same hepatocytes." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 296-300, (2007) (PubMed).

Kodai, Takemura, Minamiyama, Hai, Yamamoto, Kubo, Yoshida, Niki, Okada, Hirohashi, Suehiro: "S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats." in: Free radical research, Vol. 41, Issue 4, pp. 489-97, (2007) (PubMed).

Tanito, Kaidzu, Anderson: "Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats." in: Investigative ophthalmology & visual science, Vol. 48, Issue 4, pp. 1864-72, (2007) (PubMed).

Kurimoto, Kawano, Tsuji, Hatachi, Jikimoto, Sugiyama, Kasagi, Komori, Nakamura, Yodoi, Kumagai: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, (2007) (PubMed).

Hirono, Yoshimoto, Suzuki, Sugiyama, Sakurada, Takai, Kobayashi, Shichiri, Hirata: "Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: the possible role of local renin-angiotensin system." in: Endocrinology, Vol. 148, Issue 4, pp. 1688-96, (2007) (PubMed).

Choi, Ryu, Kim, McLarnon: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, (2007) (PubMed).

Tanito, Elliott, Kotake, Anderson: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, (2005) (PubMed).

Shiraishi, Naito: "Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele." in: Human reproduction (Oxford, England), Vol. 20, Issue 9, pp. 2608-13, (2005) (PubMed).

Nagotani, Hayashi, Sato, Zhang, Deguchi, Nagano, Shoji, Abe: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, (2005) (PubMed).

Yamada, Funada, Shibata, Kobayashi, Kawai, Tatsuda, Furuhata, Uchida: "Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids." in: Journal of lipid research, Vol. 45, Issue 4, pp. 626-34, (2004) (PubMed).

Liu, Ormsby, Oja-Tebbe, Pagano: "Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy." in: Circulation research, Vol. 95, Issue 6, pp. 587-94, (2004) (PubMed).

Tam, Gao, Leung, Ho: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, (2003) (PubMed).

Tanito, Nishiyama, Tanaka, Masutani, Nakamura, Yodoi, Ohira: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, (2002) (PubMed).

Hung, Skepper, Burton: "In vitro ischemia-reperfusion injury in term human placenta as a model for oxidative stress in pathological pregnancies." in: The American journal of pathology, Vol. 159, Issue 3, pp. 1031-43, (2001) (PubMed).

Yamagami, Yamamoto, Kume, Ishikawa, Yamaoka, Hiai, Toyokuni: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, (2001) (PubMed).

Target Details for HNE

Target: HNE (4-Hydroxynonenal (HNE))

Abstract: HNE Products

Target Type: Chemical

Background: During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.