tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE)

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517, ywhae, zgc:55377, zgc:77062, wu:fb48f06, wu:fi22e10, MGC53170

Clonality: Monoclonal (5A5)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN336074

Additional Information

Characteristics: Synonyms: 14-3-3E, YWHAE

Alternative name: 14-3-3 protein epsilon

Swiss-Prot: P62258

Immunogen: Recombinant Human 14-3-3 epsilon (1-255 aa) purified from E. coli.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 5A5

Description: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpointcontrol, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved andubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that havebeen identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins,was thought to be brain and neuron-specific. It has been shown to interact with CDC25phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activitiesrelated to signal transduction, such as cell division and regulation of insulin sensitivity. Ithas also been implicated in the pathogenesis of small cell lung cancer.

Specificity: The antibody recognizes Human and Mouse 14-3-3 epsilon. Other species not tested.

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517, ywhae, zgc:55377, zgc:77062, wu:fb48f06, wu:fi22e10, MGC53170

Application Details

Application Notes: ELISA. Western blot: 1/1,000-1/2,000. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4, containing 0.09% Sodium Azide as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Transcription Factors

Restrictions: For Research Use only