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Activating Transcription Factor 2 (ATF2) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Clonality Polyclonal
Host
Alternatives

Sheep
Reactivity
Alternatives

Cow (Bovine)
Application
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Catalog no. ABIN336137
Quantity 0.2 mg  (2.5 mg/ml)
Price 340.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, CyclicAMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2,cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-bindingprotein 2
Alternative name ATF2
Swiss-Prot P15336
Immunogen An Escherichia coli-derived GST fusion protein corresponding to the Nterminal (a. a 95-211)of human ATF2.
Cross-Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
Description The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNAresponse elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is atarget of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellularstress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulatethe transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation ofThr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 atthese sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in theloss of stress-induced transcription by ATF-2. In addition, mutations at these sites reducethe ability of E1A and Rb to stimulate gene expression via ATF-2.
Specificity This antiobody recognises human Activating Transcription Factor 2 (ATF2), a nuclearprotein that promotestranscription in the presence of cyclic AMP. Does not react with ATF-1, ATF-3. Reactive with GST, and other GST Fusion proteins. (Enquire for antibody affinity absorbedfor GST). Species: Human, Mouse, Rat, Bovine, Dog and Horse.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Immunoprecipitation (1-10 μg/ml). Western blot (1-10 ug/ml): This antibody recognises a band at approximately 65kDa inHeLa nuclear extracts. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 2.5 mg/ml
Purification Purified
Buffer PBS containing 0.09% Sodium Azide as preservative.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Immunology, Innate Immunity, Transcription Factors
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (151), Mouse (16), Goat (4), Sheep (2)
Reactivities Human (171), Mouse (Murine) (101), Rat (Rattus) (90), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2), Rabbit (2)
Applications Western Blotting (WB) (108), Immunohistochemistry (IHC) (65), Immunofluorescence (IF) (49), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Enzyme Immunoassay (EIA) (32), Immunoprecipitation (IP) (32), ELISA (24), Flow Cytometry (FACS) (19), Dot Blot (Dot) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pThr69 or 51 (5), pThr69/51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer62/44 (4), pThr71/53 (4), pSer112/94 (3), pThr73/55 (3), S44 (2), S94 (2), S322 (1), pSer112, pSer94 (1), pSer340, pSer322 (1), pSer44 (1), pSer472 (1), pSer62, pSer44 (1), pSer94 (1), pThr51 (1), pThr69, pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79, pThr51 (1)