Cytokeratin 8/18 antibody

Catalog No. ABIN336865

Product Details anti-Cytokeratin 8/18 Antibody

Target: Cytokeratin 8/18

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 8/18 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Specificity: Molecular Weight: CK 8 is52.5 kDa and CK 18 is 45 kDa.

Immunogen: Purified keratins

Clone: CK8-18 207

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. For FFPE tissue sections antigen retriever like trypsin (our catalog. # AR-6541) or citrate (our catalog # AR-6544) is required. (Citrate antigen retriever: Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. dilution should be determined by the individual lab. Positive Control: Human breast, any GI tissue or skin. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Protein A purified antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Cytokeratin 8/18

Target: Cytokeratin 8/18

Alternative Name: Cytokeratin 8+18 (Cytokeratin 8/18 Products)

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. This antibody recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract). Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative.