GJB6 (C-Term) antibody

Antigen: GJB6 (C-Term)

Clonality: Monoclonal (CX30-8E8)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN337000

Additional Information

Swiss-Prot: O95452

Immunogen: Synthetic peptide derived from the unique C-terminus of the mouse Connexin 30.

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: CX30-8E8

Description: Gene: GJB6, Standard Gene Symbol: GJB6, Family: Ion Channel, Subfamily: Connexin, Synonyms: GJB6, Connexin 30, Connexin-30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap Junction Protein Beta-6, HED

Specificity: On Western blots of lysates derived from total rat or mouse brain, this monoclonal antibody detects a protein of ~30kD corresponding to the authentic Connexin 30 (Cx30) protein. A Cx30 dimer is not detected although other unidentified bands migrating between 70-80kD may be. When used to probe Western blots of rat liver (rat liver does not contain Cx30), several unknown bands are detected at ~110, 53 and 35kD. Immunostaining of fixed, frozen adult rat brain with CX30-8E8 produces a typical punctate staining pattern in the globus pallidus/hypothalamus. Reacts with Connexin 30 from mice and rats. Predicted Reactivities: Human.

Application Details

Application Notes: ELISA (0.1 - 1 µg/ml), IF (1 - 5 µg/ml), IHC-Fr, IHC-P (2.5 µg/ml), WB (1 µg/ml)

Concentration: 0.5 mg/ml

Purification: Protein A Column

Buffer: PBS, pH 7.4, 0.1% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with GJB6 antibody ABIN337000 at 2.5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.