CES1 antibody (AA 533-544)

Catalog No. ABIN337128

Product Details anti-CES1 Antibody

Target: CES1 (Carboxylesterase 1 (CES1))

Binding Specificity: AA 533-544

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CES1. This antibody is expected to recognise all three reported isoforms (NP_001020366.1, NP_001020365.1, NP_001257.4).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Monkey (100%) Gorilla, Gibbon, Marmoset, Horse (92%) Mouse, Rat, Hamster, Panda, Bovine, Cat (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NTQAAQKLKDKE from an internal region (near C-terminus) of human CES1 (NP_001020366.1, NP_001020365.1, NP_001257.4). Percent identity by BLAST analysis: Human, Orangutan, Monkey (100%), Gorilla, Gibbon, Marmoset, Horse (92%), Mouse, Rat, Hamster, Panda, Bovine, Cat (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: Carboxylesterase 1 / CES1 (CES1 Products)

Background: Name/Gene ID: CES1

Synonyms: CES1, ACAT, Brain carboxylesterase hBr1, Carboxylesterase 1, Carboxylesterase 2 (liver), CEH, CES1A1, CES1A2, Cocaine carboxylesterase, Egasyn, HMSE, Liver carboxylesterase 1, HCE-1, PCE-1, Retinyl ester hydrolase, TGH, Triacylglycerol hydrolase, REH, SES1, CE-1, Cholesteryl ester hydrolase, HMSE1, Serine esterase 1

Gene ID: 1066

NCBI Accession: NP_001020366, NP_001020365, NP_001257

UniProt: P23141

Pathways: Monocarboxylic Acid Catabolic Process