APOB antibody (Biotin)

Antigen: APOB

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337166

Additional Information

Swiss-Prot: P04114

Immunogen: Purified protein, Apolipoprotein Type B isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Isotype: IgG

Description: Gene: APOB, Standard Gene Symbol: APOB, Synonyms: APOB, Apo b-100, Apo b-48, Apolipoprotein b-100, Apolipoprotein b-48, FLDB, LDLCQ4

Specificity: Recognizes human apolipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific cross-reaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.

Application Details

Application Notes: ELISA (1:1000 - 1:5000), IHC-P (1:25 - 1:50), IP, WB (1:1000 - 1:5000)

Purification: Immunoaffinity Chromatography

Buffer: PBS, pH 7.2. No preservative added. No stabilizing proteins added.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Human Liver (formalin-fixed, paraffin-embedded) stained with APOB antibody ABIN337166 at 1:25-1:50 followed by biotinylated anti-goat IgG secondary antibody ABIN481715, alkaline phosphatase-streptavidin and chromogen.