PC antibody (AA 886-899)

Catalog No. ABIN337183

Product Details anti-PC Antibody

Target: PC (Pyruvate Carboxylase (PC))

Binding Specificity: AA 886-899

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Monkey, Horse, Hamster, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%) Goat, Stickleback, Pufferfish (93%) Opossum, Chicken, Xenopus, Zebrafish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2, NP_001035806.1, NP_071504.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%), Goat, Stickleback, Pufferfish (93%), Opossum, Chicken, Xenopus, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: PC / Pyruvate Carboxylase (PC Products)

Synonyms: BcDNA antibody, BcDNA:GH06348 antibody, Dmel\\CG1516 antibody, PC antibody, pyc antibody, wu:fb82g08 antibody, wu:fj85g01 antibody, PCX antibody, BA4157 antibody, Pc antibody, Pcb antibody, PCB antibody, Pcx antibody, Pyruvate carboxylase antibody, pyruvate carboxylase b antibody, PYruvate Carboxylase antibody, pyruvate carboxylase antibody, pyruvate carboxylase Pyr1 antibody, PCB antibody, pcxb antibody, pyc-1 antibody, PC antibody, pyc antibody, pyr1 antibody, CNF00650 antibody, PYC1 antibody, Ndas_1152 antibody, Ilyop_2503 antibody, Calni_0495 antibody, Ftrac_1004 antibody, Plabr_3574 antibody, Sgly_2936 antibody, Celly_0558 antibody, Hipma_0131 antibody, Halhy_1567 antibody, Mesop_1269 antibody, Pcx antibody, Pc antibody

Background: Name/Gene ID: PC

Synonyms: PC, PCB, Pyruvate carboxylase, Pyruvic carboxylase

Gene ID: 5091

NCBI Accession: NP_000911, NP_001035806, NP_071504

UniProt: P11498