MMP 9 antibody

Catalog No. ABIN337212

The Rabbit Polyclonal anti-MMP 9 antibody (ABIN337212) specifically detects MMP 9 in WB, IHC, IP, IHC (p) and IHC (fro). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for MMP 9 antibody (ABIN337212)

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP 9 Antibody

Specificity: Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

Purification: Protein A purified

Immunogen: E. coli expressed active rat 92kD type IV collagenase (catalytic domain).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:50), IHC-P (5 μg/mL), IP (1:250), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP9 / Gelatinase B

Background: Name/Gene ID: MMP9
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP9, 92kd gelatinase, 92 kd type iv collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Matrix metalloproteinase-9, MMP-9, Type V collagenase, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2

Gene ID: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events