GTPase NRas antibody (AA 167-179)

Catalog No. ABIN337227

Product Details anti-GTPase NRas Antibody

Target: GTPase NRas (NRAS)

Binding Specificity: AA 167-179

Reactivity: Human, Mouse, Dog, Cow, Horse, Bat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NRAS.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%) Marmoset, Rat, Hamster, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%), Marmoset, Rat, Hamster, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS / N-ras (NRAS Products)

Synonyms: NRAS antibody, AV095280 antibody, N-ras antibody, ALPS4 antibody, NRAS1 antibody, NS6 antibody, N-RAS antibody, fj99a10 antibody, wu:fj99a10 antibody, zgc:100761 antibody, zras-b1 antibody, H-RAS antibody, HRAS antibody, GTPase NRas antibody, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antibody, NRAS proto-oncogene, GTPase antibody, neuroblastoma ras oncogene antibody, neuroblastoma RAS viral oncogene homolog antibody, Tsp_01117 antibody, nras.L antibody, Nras antibody, NRAS antibody, nras antibody

Background: Name/Gene ID: NRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: NRAS, HRAS1, NRAS1, NS6, ALPS4, Transforming protein N-Ras, N-ras, N-ras protein part 4, GTPase NRas

Gene ID: 4893

NCBI Accession: NP_002515

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling