MAP3K1 / MEKK1 (AA 130-300) antibody

Antigen: MAP3K1 / MEKK1 (AA 130-300)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337247

Additional Information

Swiss-Prot: Q13233

Immunogen: Recombinant protein encompassed within amino acids 130-300 of the human MEKK1 protein.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: MAP3K1 / MEKK1, Standard Gene Symbol: MAP3K1, Family: Protein Kinase, Subfamily: MAP3K, Synonyms: MAP3K1, MAP/ERK kinase kinase 1, MAPKKK1, Mek kinase 1, MEKK, MEKK1

Specificity: Reacts specifically with MEKK1 protein detecting a monomer species migrating at ~69kD and a trimer species migrating at ~200kD. Cross-reactivity with other proteins has not been observed. Species cross-reactivity: human MEKK1. Tested by Western blotting with HeLa, 293 (kidney) and MEKK1 transfected cells.

Application Details

Application Notes: ELISA, IHC-P (5 µg/ml), WB (1 - 3 µg/ml)

Concentration: 0.25 mg/ml

Purification: Affinity Chromatography

Buffer: PBS, pH 7.4, 0.1% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Human Lung, Alveolar Macrophages (formalin-fixed, paraffin-embedded) stained with MAP3K1 antibody ABIN337247 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen.