C3 / Complement C3 antibody

Antigen: C3 / Complement C3

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337267

Additional Information

Swiss-Prot: P01024

Immunogen: Purified protein, C3 isolated from human plasma.

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Description: Gene: C3 / Complement C3, Standard Gene Symbol: C3, Synonyms: C3, AHUS5, ARMD9, ASP, Complement c3, CPAMD1

Specificity: Recognizes Complement component C3 (human). Epitope specificity differs from that of clones 6F66 and 6F63 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against C3. In Western blotting after SDS-PAGE, this antibody reacts with C3 in reduced as well as non-reduced forms.

Application Details

Application Notes: ELISA (1:30000), IHC-P (10 µg/ml), WB

Concentration: 0.5 mg/ml

Purification: Protein A/G Column

Buffer: PBS, pH 7.2, 15 mM sodium azide, before the addition of glycerol to 40%

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Human Liver (formalin-fixed, paraffin-embedded) stained with C3 antibody ABIN337267 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.