TRIM21 antibody (AA 386-398)

Catalog No. ABIN337310

Product Details anti-TRIM21 Antibody

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Binding Specificity: AA 386-398

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM21 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM21.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CPLNIGSQGSTDY from the C-terminus of human TRIM21 (NP_003132.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM21

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Alternative Name: TRIM21 / RO52 (TRIM21 Products)

Background: Name/Gene ID: TRIM21
Family: Tripartite Motif

Synonyms: TRIM21, 52 kDa Ro protein, RING finger protein 81, RO52, Ro(SS-A), SSA1, Tripartite motif-containing 21, RNF81, SSA, Sicca syndrome antigen A, SS-A, Tripartite motif containing 21

Gene ID: 6737

NCBI Accession: NP_003132

UniProt: P19474