APOB antibody

Catalog No. ABIN337314

The Goat Polyclonal anti-APOB antibody (ABIN337314) specifically detects APOB in IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for APOB antibody (ABIN337314)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOB Antibody

Specificity: Recognizes Apo B (monospecific) by IEP.

Purification: Immunoaffinity purified

Immunogen: Affinity purified Apolipoprotein-B (hLDL).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris-HCl, 0.5 M sodium chloride,  pH 7.5, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B / APOB

Background: Name/Gene ID: APOB

Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48

Gene ID: 338

UniProt: P04114

Pathways: Lipid Metabolism