SMAD9 antibody (AA 166-179)

Catalog No. ABIN337365

Product Details anti-SMAD9 Antibody

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Binding Specificity: AA 166-179

Reactivity: Human, Mouse, Rat, Dog, Bat, Chicken, Cow, Hamster, Horse, Monkey, Pig, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SMAD9. This antibody is not expected to cross-react with SMAD1 and SMAD5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%) Opossum, Platypus, Lizard (93%) Stickleback (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HSEPLMPHNATYPD from an internal region of human SMAD9 (NP_005896.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%), Opossum, Platypus, Lizard (93%), Stickleback (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Alternative Name: SMAD9 (SMAD9 Products)

Synonyms: SMAD8 antibody, MADH6 antibody, MADH9 antibody, PPH2 antibody, SMAD8A antibody, SMAD8B antibody, Madh8 antibody, Madh9 antibody, Smad8 antibody, SMAD family member 9 antibody, SMAD9 antibody, smad9 antibody, Smad9 antibody

Background: Name/Gene ID: SMAD9

Synonyms: SMAD9, MAD homolog 9, SMAD 9, SMAD8A, SMAD8, Smad9, MADH9, SMAD8B, MADH6, Mothers against DPP homolog 9, SMAD family member 9

Gene ID: 4093

NCBI Accession: NP_005896

UniProt: O15198