KIAA0319 antibody (AA 391-403)

Catalog No. ABIN342965

Product Details anti-KIAA0319 Antibody

Target: KIAA0319

Binding Specificity: AA 391-403

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This KIAA0319 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Mouse KIAA0319. The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1), isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1).

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QGKIKQENKPTLH from an internal region of mouse KIAA0319 (NP_001074520.1). Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000, 0.5 - 1.5 μg/mL), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kD band observed in Mouse Brain lysates (calculated MW of 118kD according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15,17(6):859-71, PMID: 18063668). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 25kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KIAA0319

Target: KIAA0319

Alternative Name: KIAA0319 (KIAA0319 Products)

Synonyms: DYLX2 antibody, DYX2 antibody, 4930451E12Rik antibody, Kiaa0319 antibody, KIAA0319 antibody, RIKEN cDNA D130043K22 gene antibody, KIAA0319 ortholog antibody, similar to mKIAA0319 protein antibody, KIAA0319 antibody, D130043K22Rik antibody, RGD1307443 antibody

Background: Name/Gene ID: KIAA0319

Synonyms: KIAA0319, Dyslexia susceptibility 2, DYX2, DYLX2

Gene ID: 9856

NCBI Accession: NP_001074520

UniProt: Q5VV43