SAV1 antibody (AA 254-266)

Catalog No. ABIN342984

Product Details anti-SAV1 Antibody

Target: SAV1 (Salvador Homolog 1 (SAV1))

Binding Specificity: AA 254-266

Reactivity: Human, Pig, Rabbit, Dog, Horse, Cow, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This SAV1 antibody is un-conjugated

Application: ELISA

Specificity: Human SAV1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%) Marmoset, Mouse, Rat, Hamster, Elephant (92%) Stickleback, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DHTNKKAQYRHPC from an internal region of human SAV1 (NP_068590.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%), Marmoset, Mouse, Rat, Hamster, Elephant (92%), Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave an approx 37kD band in Human Kidney lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.6kD according to NP_068590.1. The 37kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SAV1

Target: SAV1 (Salvador Homolog 1 (SAV1))

Alternative Name: SAV1 / WW45 (SAV1 Products)

Synonyms: SAV antibody, WW45 antibody, WWP4 antibody, 1700040G09Rik antibody, Salv antibody, Sav antibody, Wwp3 antibody, Wwp4 antibody, salvador family WW domain containing protein 1 antibody, salvador family WW domain containing 1 antibody, SAV1 antibody, Sav1 antibody

Background: Name/Gene ID: SAV1

Synonyms: SAV1, 45 kDa WW domain protein, HWW45, WW domain-containing, WW45, Salvador, SAV, WWP4, Protein salvador homolog 1

Gene ID: 60485

NCBI Accession: NP_068590

UniProt: Q9H4B6

Pathways: Stem Cell Maintenance