PGBD1 antibody (AA 230-241)

Catalog No. ABIN342993

Product Details anti-PGBD1 Antibody

Target: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Binding Specificity: AA 230-241

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PGBD1 antibody is un-conjugated

Application: ELISA

Specificity: Human PGBD1.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKWSHLSLTRRN from an internal region of human PGBD1 (NP_115896.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Orangutan (83%), Monkey (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:4000)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave bands at approx 150kD and 17kD in lysates of cell lines HeLa, HepG2 and K562 after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 92.5kD according to NP_115896.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PGBD1

Target: PGBD1 (PiggyBac Transposable Element Derived 1 (PGBD1))

Alternative Name: PGBD1 (PGBD1 Products)

Synonyms: 4921509E05Rik antibody, PGBD1 antibody, DKFZp469J1439 antibody, HUCEP-4 antibody, SCAND4 antibody, dJ874C20.4 antibody, piggyBac transposable element derived 1 antibody, PGBD1 antibody, Pgbd1 antibody

Background: Name/Gene ID: PGBD1

Synonyms: PGBD1, Cerebral protein 4, Cerebral protein-4, DJ874C20.4, HUCEP-4, SCAND4

Gene ID: 84547

NCBI Accession: NP_115896

UniProt: Q96JS3