OTUD5 antibody (AA 414-426)

Catalog No. ABIN343070

Product Details anti-OTUD5 Antibody

Target: OTUD5 (OTU Domain Containing 5 (OTUD5))

Binding Specificity: AA 414-426

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This OTUD5 antibody is un-conjugated

Application: ELISA

Specificity: Human OTUD5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Horse, Rabbit (100%) Elephant (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RQVRGPSQPRKAS from an internal region of human OTUD5 (NP_060072.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Horse, Rabbit (100%), Elephant (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:64000)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 80kD band in lysates of cell lines Jurkat and MOLT4 after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.6kD according to NP_060072.1. The 80kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for OTUD5

Target: OTUD5 (OTU Domain Containing 5 (OTUD5))

Alternative Name: OTUD5 (OTUD5 Products)

Synonyms: DUBA antibody, AA407879 antibody, AI553596 antibody, BB114028 antibody, DXImx46e antibody, Sfc7 antibody, otud5 antibody, si:bz30i22.1 antibody, si:rp71-30i22.1 antibody, zgc:154072 antibody, wu:fi28a06 antibody, zgc:100839 antibody, OTU deubiquitinase 5 antibody, OTU domain containing 5 antibody, OTU deubiquitinase 5a antibody, OTU deubiquitinase 5 S homeolog antibody, OTU deubiquitinase 5 L homeolog antibody, OTU deubiquitinase 5b antibody, OTUD5 antibody, Otud5 antibody, otud5a antibody, otud5.S antibody, otud5.L antibody, otud5b antibody

Background: Name/Gene ID: OTUD5

Synonyms: OTUD5, Deubiquitinating enzyme A, Deubiquinating enzyme A, DUBA, OTU domain containing 5

Gene ID: 55593

NCBI Accession: NP_060072

UniProt: Q96G74