CD14 antibody (AA 9-13, AA 39-44)

Catalog No. ABIN343168

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 9-13, AA 39-44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Agonist (Agon)

Specificity: Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

Cross-Reactivity: Corynebacterium diphtheriae, Cow (Bovine), Dog (Canine), Horse (Equine), Pig (Porcine)

Cross-Reactivity (Details): CD14 of cattle++, swine ++, dog+ horse+

Purification: Protein G affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Clone: BiG13

Isotype: IgG1

Application Details

Application Notes: 20 μg/mL inhibits binding of 0.5 μg/ ml LPS to CD14.Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.5

Preservative: Without preservative

Storage: -20 °C,-80 °C

References for anti-CD14 antibody (ABIN343168)

Moyo, Marchi, Steinbach: "Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression." in: Immunology, Vol. 139, Issue 4, pp. 472-83, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Kato, Ogasawara, Homma, Saito, Matsumoto: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, (2004) (PubMed).

Berner, Fürll, Stelter, Dröse, Müller, Schütt: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, (2002) (PubMed).

Vidal, Labéta, Schiffrin, Donnet-Hughes: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, (2001) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: Background: biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molecular Weight: ~50kDa

Gene ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades