CD14 antibody (CD14 Molecule)

Details for Product anti-CD14 Antibody No. ABIN343168, Supplier: Log in to see
Antigen
  • CD14 molecule
  • CD14 antigen
  • CD14
  • Cd14
Alternatives
anti-Human CD14 antibody for Biochemical Assay
Reactivity
Human
954
151
115
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98
79
71
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63
59
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40
34
32
27
17
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13
9
9
7
3
2
1
1
1
Host
Mouse
894
125
68
23
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD14 antibody is un-conjugated
107
101
71
48
32
28
27
24
21
19
15
15
15
15
15
15
10
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10
8
8
8
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7
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2
2
2
2
1
1
1
1
1
1
1
1
1
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1
Application
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
880
321
276
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138
80
70
63
60
14
13
13
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9
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3
3
3
3
2
2
2
2
1
1
1
1
Options
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Immunogen Monocytes of different species and immunoaffinity purified soluble human CD14
Clone BiG 13
Isotype IgG1
Purification Protein G purified
Alternative Name CD14 (CD14 Antibody Abstract)
Background BiG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Application Notes Suitable for Immunostaining of CD14 positive cells (flow cytometry), ELISA, CD14 inhibition studies, Western Blot. 20ug/ml inhibits binding of 0.5 ug/ ml LPS to CD14, binding epitopes amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+ Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000 Inhibits LPS induced IL1
Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/ml
Buffer 0.1mg in PBS without NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-CD14 antibody (CD14 Molecule) (ABIN343168) anti-CD14 Molecule (CD14) antibody
Product cited in: Moyo, Marchi, Steinbach: "Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression." in: Immunology, Vol. 139, Issue 4, pp. 472-83, 2013 (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, 2012 (PubMed). Further details: Monoclonal anti-CD14 antibody (anti-CD14 mAb, biG14) was used to block the CD14 receptor for LPS

Kato, Ogasawara, Homma, Saito, Matsumoto: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, 2004 (PubMed).

Berner, Fürll, Stelter, Dröse, Müller, Schütt: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, 2002 (PubMed).

Vidal, Labéta, Schiffrin, Donnet-Hughes: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, 2001 (PubMed).