CD14 antibody (AA 39-41, AA 43-44)

Catalog No. ABIN343169

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 39-41, AA 43-44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Soluble and membrane bound human CD14 The antibody blocks LPS binding to membrane bound CD14

Cross-Reactivity: Corynebacterium diphtheriae, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Pig (Porcine)

Cross-Reactivity (Details): cattle++, swine ++, dog+ horse+, guinea pig +,Inhibits binding of LPS to CD14 of swine++, dog+, cattle +, dog+, more than titer 10.000.

Purification: Protein A affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Clone: BiG14

Isotype: IgG1

Application Details

Application Notes: binding titre at human CD14 transfected CHO-cells: 1:50.000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.6

Preservative: Without preservative

Storage: -20 °C,-80 °C

References for anti-CD14 antibody (ABIN343169)

Knobloch, Feldmann, Wahl, Jungck, Behr, Stoelben, Koch: "Endothelin receptor antagonists attenuate the inflammatory response of human pulmonary vascular smooth muscle cells to bacterial endotoxin." in: The Journal of pharmacology and experimental therapeutics, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Schromm, Reiling, Howe, Wiesmueller, Roessle, Brandenburg: "Influence of serum on the immune recognition of a synthetic lipopeptide mimetic of the 19-kDa lipoprotein from Mycobacterium tuberculosis." in: Innate immunity, (2009) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: Background: biG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molecular Weight: ~50kDa

Gene ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades