Cullin 4B (CUL4B) antibody

Antigen: Cullin 4B (CUL4B)

Synonyms: SFM2, MRXSC, MRXHF2, KIAA0695, DKFZp686F1470, AA409770, mKIAA0695, 2700050M05Rik, RGD1564494, im:7140950, im:7150765, MGC115611, Tb03.1J15.620, sfm2, mrxsc, mrxhf2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Zebrafish (Danio rerio), Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343367

Additional Information

Alternative name: Cullin 4B, CUL4B

Immunogen: A synthetic peptide corresponding to a sequence of human Cullin 4B, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. 1 The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). 2 Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex. 3

Specificity: Zebrafish, human. No cross reactivity with other proteins.

Application Details

Application Notes: WB 1ug/ml (Zebrafish, Hu). IHC-P 2ug/ml (Zebrafish). Antigen Retrieval by heat

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only