Deoxynucleotidyltransferase, terminal (DNTT) antibody

Antigen: Deoxynucleotidyltransferase, terminal (DNTT)

Synonyms: TDT, Tdt, BB160593, DNTT, MGC109152, dntt-A, TdT

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343373

Additional Information

Alternative name: Terminal Deoxynucleotidyl Transferase, TDT

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human TDT, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. 1 Its gene is mapped to the region 10q23-q24. 2 And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. 3 TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Specificity: Human. No cross reactivity with other proteins.

Application Details

Application Notes: WB 1ug/ml (Hu)

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only