Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8) antibody

Antigen: Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343382

Additional Information

Alternative name: Fibroblast growth factor 8, FGF8

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human FGF8, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Fibroblast growth factor 8 (androgen-induced), also known as FGF8 or AIGF, is a human gene which maps to 10q24. The protein encoded by this gene are secreted proteins that interact with FGF tyrosine kinase receptors to mediate growth and development. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The temporal and spatial patterns of this gene expression suggest that FGF8 is involved in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. The adult expression of FGF8 is restricted to gonads, including testes and ovaries. FGF8 stimulated growth of human prostate carcinoma cells and mouse fibroblasts and mammary carcinoma cells in a dose-dependent manner. 1 It also may play an important role in growth and patterning of limbs, face, and central nervous system. 2 FGF8 is expressed in increased levels in breast cancer and in lactating human breast, it was also detected in human milk. A survey of other normal tissues showed that FGF8 is expressed in the proliferative cells of the skin and epithelial cells in colon, ovary, fallopian tube, and uterus. 3

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 1µg/ml (Hu, Rat, Ms). IHC-P 1-2µg/ml (Hu, Rat, Ms). Antigen Retrieval by heat

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Restrictions: For Research Use only