Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3) antibody

Antigen: Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3)

Synonyms: P2X3, MGC129956, MGC28163, P2RX3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343385

Additional Information

Alternative name: Purinergic receptor P2X, ligand-gated ion channel 3, P2X3

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human P2X3, different from the related rat sequence by one amino acid.

Format: Affinity-purified

Isotype: IgG

Description: Purinergic receptor P2X, ligand-gated ion channel, 3, also known as P2X3, is a human gene. Its gene is mapped to 11q12. P2X purinoceptors are 397 to 492 amino acids long and have a predicted structure of 2 short intracellular domains, 2 transmembrane-spanning regions, and a large extracellular domain. The P2X3 subunit has 43% and 47% identity with P2X1 and P2X2, respectively, 10 cysteine residues are conserved in all 3 subtypes, so that tertiary structures may also be conserved. 1 P2X3 was the only ligand-gated channel known to be expressed exclusively by a subset of sensory neurons. The remarkable selectivity of expression of the channel, coupled with a sensory neuron-like pharmacology, suggested that it may transduce ATP-evoked nociceptor activation. 2 If ATP, and more specifically, P2X3 purinoceptors are involved in nociception, then the development of an antagonist selective for P2X3 could prove useful in pain relief. Lack of P2X3 in other tissues than sensory ganglia could afford a degree of specificity, leading to fewer side effects. 3

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 1ug/ml (Hu, Rat, Ms). IHC-P 1-2ug/ml (Rat, Ms). Antigen Retrieval by heat

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only