Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) antibody

Antigen: Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8)

Synonyms: HNC, CLG1, PMNL-CL, BB138268, MMP8

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343390

Additional Information

Alternative name: Matrix Metalloproteinase 8, MMP8

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human MMP8, different to the related rat sequence by three amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: MMP8 (Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases. It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8 was mapped to 11q21-q22. MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. 1 The enzyme exhibits 58% homology to human fibroblast collagenase and has the same domain structure. It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond. 2 MMP8 was found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. 3 Matrix metalloproteinases (MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer. MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility. 4

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 0.75ug/ml (Hu, Rat, Ms). IHC-P 1-2ug/ml (Hu, Rat, Ms)

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only