Growth Hormone Receptor (GHR) antibody

Antigen: Growth Hormone Receptor (GHR)

Synonyms: GHBP, GHR/BP, AA986417, MGC124963, MGC156665, GHR, LOC398153, Ghr, ghr

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343391

Additional Information

Alternative name: Growth Hormone Receptor, GHR

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human GHR, different to the related rat sequence by a single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The GHR locus to human chromosome 5p13.1-p12 and to mouse chromosome 15. 1 Additionally, its gene has 9 exons that encode the receptor and several additional exons in the 5-prime untranslated region. The coding exons span at least 87 kb. 2 GHR consists of an extracellular domain of 246 amino acids, a single transmembrane domain, and a cytoplasmic domain. Exons 3 to 7 encode the extracellular domain. There are 2 isoforms of GHR in humans, generated by retention or exclusion of exon 3 during splicing: a full-length isoform and an isoform that lacks exon 3 (d3GHR). Furthermore, the two isoforms of GHR are expressed in the placenta and appeared to be due to alternative splicing. In cirrhosis, there is a state of acquired GH resistance, as defined by high circulating GH levels with low IGF1 levels. Moreover, Mutations in the GHR gene have been demonstrated as the cause of Laron syndrome , also known as the growth hormone insensitivity syndrome (GHIS). 3

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 0.75ug/ml (Hu, Rat, Ms). IHC-P 1-2ug/ml (Hu). Antigen Retrieval by heat

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only