Insulin Receptor alpha antibody

Antigen: Insulin Receptor alpha

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343392

Additional Information

Alternative name: Insulin Receptor alpha, Insulin Ralpha

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human Insulin Ralpha, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Insulin receptor is a tetramer of 2 alpha and 2 beta subunits. The alpha and beta subunits are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. The insulin receptor has an intrinsic tyrosine kinase activity that is essential for signal transduction. A mutant insulin receptor gene lacking almost the entire kinase domain has been identified in an individual with type A insulin resistance and acanthosis nigricans. 1

Specificity: Human, mouse, rat, dog. No cross reactivity with other proteins.

Application Details

Application Notes: WB 0.75µg/ml (Hu, Rat, Ms). IHC-P 1-2µg/ml (Dog, Rat, Ms)

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at +4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Restrictions: For Research Use only