CHK2 Checkpoint Homolog (S. Pombe) (CHEK2) antibody

Antigen: CHK2 Checkpoint Homolog (S. Pombe) (CHEK2)

Synonyms: CDS1, CHK2, LFS2, RAD53, HuCds1, PP1425, Cds1, Rad53, HUCDS1, Chk2, fa66f08, zgc:55865, wu:fa66f08, CHEK2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN343395

Additional Information

Alternative name: CHK2 Checkpoint homolog, CHK2

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human CHK2, different to the related rat sequence by a single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest. Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1 1 . Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment 2 . Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells 3 . Morever, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast 4 . There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: WB 0.75ug/ml (Hu, Rat, Ms)

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only