Corticotropin Releasing Hormone (CRH) antibody

Antigen: Corticotropin Releasing Hormone (CRH)

Synonyms: CRH, crf, zgc:101864, crh, MGC159486, CRF, Gm1347, MGC151298, crhb

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343400

Additional Information

Alternative name: Corticotropin releasing hormone, CRH (CRF)

Immunogen: A synthetic peptide mapping at the N-terminal of human CRH, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: CRH is a 41-amino acid peptide derived by enzymatic cleavage from a 191-amino acid preprohormone and is located on 8q13. It is made not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes, and is expressed in very large amounts in the human placenta. As a placental clock, CRH controls the duration of pregnancy and that the timing of the rise in CRH may permit prediction of the onset of labor. Furthermore, CRH is the most proximal element of the HPA axis, and it acts as a central coordinator for neuroendocrine and behavioral responses to stress. In addition, placental secretion of CRH is a marker of the 'placental clock' that is active from an early stage in human pregnancy and determines the length of gestation and the timing of parturition and delivery.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1µg/ml with the appropriate system to detect CRH in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1µg/ml to detect CRH in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) Suitable Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only