Estrogen Receptor 2 (ER Beta) (ESR2) antibody

Antigen: Estrogen Receptor 2 (ER Beta) (ESR2)

Synonyms: Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA, ER[b], Estrb, ERbeta, Erb2, ER-beta, ESR2, ERB, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb, esrb, estrb, nr3a2, er-beta, esr-beta, CERB, ERBETA, er, esr2, erb2, erb1, erbeta, Esr2, LOC100304489

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN343402

Additional Information

Alternative name: Estrogen receptor beta, ER beta

Immunogen: A synthetic peptide mapping at the N-terminal of human ER beta, different from the related mouse sequence by four amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Estrogen receptor-beta, referred to as ESR2, is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb. ESR2 is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells1. ESR-beta is homologous to the previously identified ESR-alpha and has an overlapping but nonidentical tissue distribution. The DNA-binding domain of ESR-beta is 96% conserved compared to ESR, and the ligand-binding domain shows 58% conserved residues. ESR-beta is expressed in human thymus, spleen, ovary, and testis2. Rat ESR-beta is expressed in rat prostate and ovary and is homologous to rat ESR (95% conserved DNA-binding domain, 55% conserved ligand-binding domain)3. ESR2 mRNA was coexpressed with ESR1 and its splice variants in 60% of prolactinomas, 100% of mixed growth hormone /prolactin tumors, and 29% of gonadotroph tumors. ESR2 gene expression was not limited to ESR1-positive tumor subtypes, however, and was also found in 100% of null cell tumors, 80% of somatotroph tumors, and 60% of corticotroph tumors4.

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Synonyms: Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA, ER[b], Estrb, ERbeta, Erb2, ER-beta, ESR2, ERB, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb, esrb, estrb, nr3a2, er-beta, esr-beta, CERB, ERBETA, er, esr2, erb2, erb1, erbeta, Esr2, LOC100304489

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect ER beta in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only