Integrin beta 2 (ITGB2) antibody

Antigen: Integrin beta 2 (ITGB2)

Synonyms: 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527, CD18, MFI7, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, ITGB2, integrin, ITGB3, MGC116306

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343404

Additional Information

Alternative name: Integrin beta2

Immunogen: A synthetic peptide mapping at the N-terminal of human Integrin beta2, different from the related mouse sequence by five amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The 3 alpha integrin chains associated individually with the beta-2 chain as a heterodimer have gene designations of ITGAL, ITGAM, and ITGAX, and leukocyte antigen designations of CD11A, CD11B, and CD11C, respectively.The expression of CD18 was increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21 1 .The ITGB2 gene spans approximately 40 kb and contains 16 exons and all exon/intron boundaries conform to the GT/AG splicing consensus 2 . Furthermore,ITGB2 was constitutively clustered. Although it was expressed on the cell surface at normal levels and was capable of function following extracellular stimulation, it could not be activated via the “inside-out” signaling pathways 3 .

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect Integrin beta2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect Integrin beta2 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only