Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) antibody

Antigen: Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343405

Additional Information

Alternative name: MMP16

Immunogen: A synthetic peptide mapping at the C-terminal of human MMP16, identical to the related rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21 1 . Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17 2 . Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms 2 . MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle 2 .

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect MMP16 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect MMP16 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1u/ml to detect MMP16 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Angiogenesis, Matrix Metalloproteinases, Extracellular Matrix

Restrictions: For Research Use only