MMP-2 antibody

Antigen: MMP-2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Catalog no.: ABIN343406

Additional Information

Alternative name: MMP2

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human MMP2, identical to the related rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase 1 and is a member of a group of secreted zinc metalloproteases 2 . The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp 3 , located within a region of human chromosome 16q13. In addition,The extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase 2 . MMP2,which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain) 5 , is functionally associated on the surface of angiogenic blood vessels 6 . NOT only is a likely effector of endometrial menstrual breakdown 4 , MMP2 is also effector and regulator of the inflammatory response 7 . Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis 8 .

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1u/ml with the appropriate system to detect MMP2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect MMP2 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 u/ml to detect MMP2 in formalin or acetone fixed tissues. Immunocytochemistry At 1-2u/ml to detect MMP2 in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Cancer, Proteolysis / Ubiquitin, Metalloprotease, Angiogenesis, Matrix Metalloproteinases, Extracellular Matrix

Restrictions: For Research Use only