Heat Shock Protein 27, HSP27 antibody

Antigen: Heat Shock Protein 27, HSP27

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343407

Additional Information

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human HSP27, different from the related mouse sequence by two amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins. Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene, which is mapped to 7q11.23 and has 3 exons 1 , produced a 2.2-kb transcript in an in vitro transcription assay. Decreasing ROS in cells expressing mutant huntingtin, HSP27 protects cells against oxidative stress 2 . In other words, HSP27 is a suppressor of polyglutamine (polyQ)-mediated cell death 3 . Furthermore, MAPKAPK5 is a major stress-activated kinase that can phosphorylate HSP27 in vitro 4 .

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1u/ml with the appropriate system to detect HSP27 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect HSP27 in formalin fixed and paraffin embedded tissues. Bioling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell lysis using P27 antibody

Immunohistochemical analysis of paraffin-embedded Intestine cancer sections. Staining P27 in nucleusDAB chromogenic reaction