Fas antibody

Antigen: Fas

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343409

Additional Information

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of rat FAS, different from the related mouse sequence by seven amino acids.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Specificity: Mouse, rat No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1u/ml with the appropriate system to detect FAS in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect FAS in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2u/ml to detect FAS in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only