Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) antibody

Antigen: Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1)

Synonyms: CPE1, CYP2E, P450-J, P450C2E, Cyp2e, CYP2E1, CYPIIE1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343412

Additional Information

Alternative name: Cytochrome P450, family 2, subfamily E, polypeptide 1, P450II E1(CYP2E1)

Immunogen: A synthetic peptide mapping at the C-terminal of human P450 E1 â…¡ , different from the related mouse sequence by single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: P450IIE1, also known as CYP2E1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man 1 . Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively 2 . In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity 3 . P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans，and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect P450 E1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect P450 E1 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) At 1-2u/ml to detect P450 E1 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only