Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) antibody

Antigen: Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14)

Synonyms: MMP14, MMP-14, mmp-x1, mtmmp1, mt1-mmp, MGC69200

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343413

Additional Information

Alternative name: MMP14

Immunogen: A synthetic peptide mapping at the C-terminal of human MMP14 different from the related mouse sequence by single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Matrix metalloproteinases (MMPs) are Zn(2+)-binding endopeptidases that degrade various components of the extracellular matrix (ECM). The MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMPs have different substrate specificities and are encoded by different genes. membrane-type matrix metalloproteinase (MMP14) may be an activator of pro-gelatinase A (MMP2) and is expressed in fibroblast cells during both wound healing and human cancer progression. Survivin, MMP2, MMP9, and MMP14 mRNA expression levels in clinically aggressive pigmented lesions were significantly higher than those in normal eutopic endometrium, and survivin gene expression in pigmented lesions was also higher than that in nonpigmented lesions (P less than 0.05). There was a close correlation between survivin and MMP2, MMP9, and MMP14 gene expression levels in 63 endometriotic tissues examined (P less than 0.01).

Specificity: Human, rat, mouse. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect MMP14 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 u/ml to detect MMP14 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 u/ml to detect MMP14 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Cancer, Proteolysis / Ubiquitin, Metalloprotease, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases

Restrictions: For Research Use only