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Antibodies
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anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibody
Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibody
| Antigen |
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| Synonyms |
ICH1, NEDD2, CASP-2, ICH-1L, ICH-1L/1S, ICH-1, Nedd2, Caspase-2, xCaspase-2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (48), Flow Cytometry (FACS) (22), Immunofluorescence (IF) (21), ELISA (19), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN343415 |
| Quantity |
100ug |
| Price |
283.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Caspase 2
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Immunogen
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A synthetic peptide mapping at the C-terminal of human Caspase 2, identical to the related rat sequence.
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Format
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Affinity-purified
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.
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Specificity
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Human, rat, mouse. No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 0.5-1u/ml with the appropriate system to detect Caspase 2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 u/ml to detect Caspase 2 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.
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Purity
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Immunogen affinity purified.
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Buffer
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Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Research Area
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Apoptosis/Necrosis, Proteolysis / Ubiquitin
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Restrictions
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For Research Use only
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Alternatives for antigen "Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2)", type "Antibodies"
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Hosts
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Rabbit (50), Mouse (13), Rat (4)
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Reactivities
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Human (64), Mouse (Murine) (32), Rat (Rattus) (26), Cow (Bovine) (16), Horse (Equine) (15), Pig (Porcine) (15), Rabbit (15), Dog (Canine) (4)
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Applications
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Western Blotting (WB) (48), Flow Cytometry (FACS) (22), Immunofluorescence (IF) (21), ELISA (19), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (2), Ab-157 (1), Center (1), cleaved (1), p18 Cleaved-Gly170 (1), p18 Cleaved-Thr325 (1), pSer157 (1)
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