Intercellular Adhesion Molecule 1 (ICAM1) antibody

Antigen: Intercellular Adhesion Molecule 1 (ICAM1)

Synonyms: BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343417

Additional Information

Alternative name: CD54 (ICAM-1)

Immunogen: A peptide mapping at the C-terminal region of the human ICAM-1. identical to the related rat and mouse sequence Purity

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: CD54, also known as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. ICAM1 protein is the major human rhinovirus receptor. ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).

Specificity: Human No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2µg/ml with the appropriate system to detect CD54 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2µg/ml to detect CD54 in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell lysis using CD54 antibody

Immunohistochemical analysis of paraffin-embedded Mammary cancer sections. Staining CD54 in cytoplasmDAB chromogenic reaction