Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) antibody

Antigen: Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)

Synonyms: APRF, HIES, FLJ20882, MGC16063, fc15d02, fl59g06, z-Stat3, MGC193054, wu:fc15d02, wu:fl59g06, STAT3, DKFZp469E1221

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343418

Additional Information

Alternative name: STAT3

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human STAT3, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21, it contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect STAT3 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect STAT3 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation

Restrictions: For Research Use only