Heat Shock Protein 60 (HSP60) antibody

Antigen: Heat Shock Protein 60 (HSP60)

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN343420

Additional Information

Alternative name: HSP60

Immunogen: A synthetic peptide mapping at the middle region of human HSP60, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Application Details

Application Notes: Western blotting: At 0.5-1u/ml with the appropriate system to detect HSP60 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect HSP60 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) Suitable Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Research Area: Heat Shock Proteins

Restrictions: For Research Use only