Orexin Receptor antibody

Antigen: Orexin Receptor

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343426

Additional Information

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Orexin Receptor, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively.Stimulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 2u/ml with the appropriate system to detect Orexin Receptor in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect Orexin Receptor in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only