Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody

Antigen: Signal Transducer and Activator of Transcription 1, 91kDa (STAT1)

Synonyms: ISGF-3, STAT91, DKFZp686B04100, AA408197, 2010005J02Rik, IMUP, H2RSP, IMUP-1, IMUP-2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343432

Additional Information

Alternative name: Signal Transducer Activator of transcription 1 (P91), STAT1 (P91)

Immunogen: A peptide mapping at the C-terminus of human stat1(P91), identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Chen et al. (1998) determined the crystal structure of the DNA complex of a 67-kD core fragment of the STAT1 homodimer, lacking only the N-domain and the C-terminal transcriptional activation domain, at 2.9-angstrom resolution. Phosphorylation of Signal Transducer and Activator of transcription 1(STAT 1) was also decreased in rheumatoid arthritis lymphocytes. The transcription factor signal transducer and activator of transcription-1 (STAT1) plays a key role in immunity against mycobacterial and viral infections. Activation of the signal transducers and activators of transcription (STAT) pathway is important in fibroblast growth factor (FGF) modulation of chondrocyte proliferation and endochondral bone formation during embryogenesis.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2ug/ml with the appropriate system to detect stat1(P91) in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2ug/ml to detect stat1(P91)in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na 2 HPO 4 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of Human carcinoma of colon tissue lysis using STAT1 antibody

Immunohistochemical analysis of paraffin-embedded Human carcinoma ventriculi sections. Staining STAT1 in cytoplasmDAB chromogenic reaction