Suppressor of Cytokine Signaling 1 (SOCS1) antibody

Antigen: Suppressor of Cytokine Signaling 1 (SOCS1)

Synonyms: JAB, CIS1, SSI1, TIP3, CISH1, SSI-1, SOCS-1, Cish1, Socs-1, zgc:91868, SOCS1, jab, cis1, ssi1, tip3, cish1, ssi-1, socs-1, socs1, LOC100228306, socs1b

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN343433

Additional Information

Alternative name: Suppressor of cytokine signaling 1, SOCS1

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Suppressor of cytokine signaling 1 (SOCS1), also known as Jak-binding protein (JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211-amino acid protein has a molecular mass of about 23.5 kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. 1 The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13. 2 SOCS1 regulates the JAK/STAT signal-transduction pathway. 3 Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis. 4

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect SOCS1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect SOCS1 in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (lung)Staining SOCS1 in nucleusDAB chromogenic reaction