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Antibody
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anti-S-adenosylmethionine decarboxylase proenzyme SAMDC
S-adenosylmethionine decarboxylase proenzyme, SAMDC antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Rabbit
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Reactivity
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Human, Mouse (Murine), Rat (Rattus)
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Application
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
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| Catalog no. |
ABIN343437 |
| Quantity |
100ug |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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A synthetic peptide corresponding to a sequence at the C-terminal of human SAMDC, identical to the related mouse and rat sequence.
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Format
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Affinity-purified
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Isotype
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IgG (Matching secondary antibodies)
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Description
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S-adenosylmethionine decarboxylase (AdoMet-DC), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC), is a key enzyme in polyamine biosynthesis. It is localized to chromosome region 6q21-q22. SAMDC has an unusual distribution in polysomes from cells of T lymphocyte origin. It associates predominantly with monosomes and small polysomes with none located in the preribosomal or ribonucleoprotein pool. SAMDC is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target. Since SAMDC is a key regulatory enzyme in the synthesis of spermidine and spermine, the marked increase in SAMDC activity in the neonate and the sustained high enzyme levels throughout adulthood, imply a role for these polyamines in both development and mature brain function.
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Specificity
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Human, mouse, rat. No cross reactivity with other proteins.
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Application Notes
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Western blotting: At 1-2u/ml with the appropriate system to detect SAMDC in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect SAMDC in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1u/ml to detect SAMDC in formalin or acetone fixed tissues. Immunocytochemistry At 2-3u/ml to detect SAMDC in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user.
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Purity
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Immunogen affinity purified.
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Buffer
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Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .
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Storage
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At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Restrictions
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For Research Use only
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