Advanced Glycosylation End Product-Specific Receptor (AGER) antibody

Antigen: Advanced Glycosylation End Product-Specific Receptor (AGER)

Synonyms: RAGE, MGC22357, AGER, MGC140817

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN343438

Additional Information

Alternative name: Advanced glycosylation end product-specific receptor, AGER (RAGE)

Immunogen: A synthetic peptide corresponding to a sequence mapping at the N-terminal of human advanced glycosylation end-product-specific receptor(RAGE), identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect RAGE in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect RAGE in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Restrictions: For Research Use only

Images

Western blot analysis of rat lung tissue lysis using RAGE antibody

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (lung)Using RAGE antibody