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Protein Phosphatase 2A Catalytic, PP2A antibody
| Antigen | Protein Phosphatase 2A Catalytic, PP2A |
| Clonality | Polyclonal |
| Host |
Rabbit |
| Reactivity |
Human, Mouse (Murine), Rat (Rattus) |
| Application |
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. | ABIN343439 |
| Quantity | 100ug |
| Price | 283.33 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Immunogen | A synthetic peptide corresponding to a sequence mapping near the N-terminal of human PP2A, identical to the related rat and mouse sequence. |
| Format | Affinity-purified |
| Isotype | IgG (Matching secondary antibodies) |
| Description | The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases. |
| Specificity | Human, mouse, rat. No cross reactivity with other proteins. |
Application Details
| Application Notes | Western blotting: At 1-2u/ml with the appropriate system to detect PP2A in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect PP2A in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry At 0.5-1u/ml to detect PP2A in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user. |
| Purity | Immunogen affinity purified. |
| Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 . |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Restrictions | For Research Use only |
