Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343444

Additional Information

Alternative name: P27

Immunogen: A peptide mapping at the C-terminal end of P27 of human origin, differs from the related rat sequence by single amino acid.

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.

Specificity: Human, mouse, rat. No cross reactivity with other proteins.

Application Details

Application Notes: Western blotting: At 1-2u/ml with the appropriate system to detect P27 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect P27 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Purity: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of Hela cell lysis using P27 antibody

Immunohistochemical analysis of paraffin-embedded Intestine cancer sections. Staining P27 in nucleusDAB chromogenic reaction